Fig. 4: HFE2 and RGMa have opposite effects on BCB integrity.
From: The liver and muscle secreted HFE2-protein maintains central nervous system blood vessel integrity
a 3D rendered representative light-sheet microscopic images of RGMa and RGMa+ HFE2 treated brains (scale bar, 400 µm) and quantification (mean ± s.e.m.; unpaired two-tail t-test; PBS n = 3, RGMa n = 5, RGMa+Hfe2 n = 4). b Representative in-vivo multiphoton images from the cerebral cortex of RGMa and RGMa+ HFE2 treated animals. The normalized extravascular fluorescence (ΔF/F) intensity was plotted over time (mean ± s.e.m.; unpaired two-tail t-tests) and the group differences were assessed with spatial leakage log-likelihood (repeated measures factorial ANOVA rendered as approximate unpaired two-tail t-test; PBS n = 6, RGMa n = 5, RGMa+ HFE2 n = 7). c Representative confocal images of fibrinogen disposition around the vessels of the cerebral cortex (isolectin) of RGMa and RGMa+ HFE2 treated mice and quantification (mean ± s.e.m.; unpaired two-tail t-test; PBS n = 5, RGMa n = 5, RGMa+ HFE2 n = 3). *P < 0.05, **P < 0.01, ***P < 0.001. Source data are provided as a Source Data file (includes exact p-values).
