Table 1 Antibodies
From: The liver and muscle secreted HFE2-protein maintains central nervous system blood vessel integrity
Primary Antibody | Species | Vendor, Catalog | RRID | Dilution |
|---|---|---|---|---|
Hfe2 | Goat, polyclonal | R&D, AF3720 | AB_2264104 | 1:200 |
CD31 | Goat, polyclonal | R&D, AF3628 | AB_2161028 | 1:100 |
Neogenin | Rabbit, polyclonal | Santa Cruz, H-175 | AB_10609239 | 1:100 |
Neogenin | Goat, polyclonal | Santa Cruz, C-20 | AB_630839 | 1:100 |
Isolectin | Griffonia simplicifolia | Invitrogen, I21411 | AB_2314662 | 1:200 |
Fibrinogen | Rabbit, polyclonal | Abcam, ab34269 | AB_732367 | 1:200 |
RGMa | Goat, polyclonal | Santa Cruz, Y-13 | AB_2269554 | 1:100 |
RGMa | Goat, polyclonal | R&D, AF2459 | AB_355273 | 1:200 |
Transferrin | Mouse, monoclonal | Santa Cruz, F-8 | AB_2077957 | 1:1000 |
CD3 | Hamster, monoclonal | BioLegend, 145-2C11 | AB_312666 | 1:100 |
B220 | Rat, monoclonal | BioLegend, RA3-6B2 | AB_10933424 | 1:200 |
CD11b | Rat, monoclonal | BioLegend, M1/70 | AB_312784 | 1:200 |
CD4 | Rat, monoclonal | BioLegend, GK1.5 | AB_312686 | 1:200 |
Claudin-5 | Rabbit, polyclonal | Abcam, ab15106 | AB_301652 | 1:300 |
GAPHD | Mouse, monoclonal | Abcam, ab8245 | AB_2107448 | 1:500 |
CD13 | Goat, polyclonal | R&D, AF2335 | AB_2227288 | 1:250 |
HIF1a | Rabbit, polyclonal | Invitrogen, 16H4L13 | 1:1000 | |
Occludin | Rabbit, polyclonal | Invitrogen, 71-1500 | AB_88065 | 1:1000 |
PLVAP | Rabbit, polyclonal | Cell signalling, 38238 | 1:1000 | |
YAP | Rabbit, polyclonal | Cell signalling, 4912 | 1:1000 | |
P-AKT | Rabbit, polyclonal | Invitrogen, 44-625 | 1:1000 | |
pSMAD1/5/9 | Rabbit, polyclonal | Cell Signaling, 13820 | 1:1000 | |
Secondary Antibody | Fluorophore | Vendor, Catalog | RRID | Dilution |
Donkey anti-goat IgG | Alexa Fluor 488 | Invitrogen, A11055 | AB_2534102 | 1:1000 |
Donkey anti-goat IgG | Alexa Fluor 594 | Invitrogen, A11058 | AB_2534105 | 1:1000 |
Donkey anti-goat IgG | Alexa fluor 647 | Invitrogen, A-21447 | AB_141844 | 1:500 |
Donkey anti-rabbit IgG | Alexa Fluor 594 | Invitrogen, A21207 | AB_141637 | 1:1000 |
Donkey anti-rabbit IgG | Alexa Fluor 488 | Invitrogen, A21206 | AB_2556546 | 1:1000 |
Goat anti-mouse IgG | IRDye 800 | LI-COR, 925-32210 | AB_2687825 | 1:10,000 |
Goat anti-rabbit IgG | IRDye 680 | LI-COR, 925-68071 | AB_10956166 | 1:10,000 |
Donkey anti-rat IgG | Alexa Fluor 488 | Invitrogen, A21208 | AB_2535794 | 1:1000 |
Donkey anti-rat IgG | Alexa Fluor 594 | Invitrogen, A21209 | AB_2535795 | 1:1000 |
