Fig. 4: LACV does not induce muscle cell death.
From: Skin muscle is the initial site of viral replication for arboviral bunyavirus infection
Mouse brain, skin, and ipsilateral quadricep (thigh) muscle (A) or ex vivo skin muscle (B) was stained with antibodies against LACV (magenta) or active caspase-3 (white). In (A) arrowheads indicate LACV infected cells, asterisks demarcate auto-fluorescent hair follicles, and dotted line shows boundaries between dermis, hypodermis, and panniculus carnosus. In (B) arrowheads indicate active caspase-3 positive staining. Scale bars represent 50 μm. OB Olfactory bulb. IC denotes region of immune cell accumulation (A, middle row). Viability of SMEVs was assessed by PrestoBlue assay to measure mitochondrial function every 2 days in mock and LACV infected tissues (C). Micrographs are representative of n = 2 (A, brain), 3 (B), or 4 (A skin, thigh) biological replicates. C Means are plotted with SD; n = 10 biological replicates per time point from two independent experiments. Source data are provided as a Source Data file.
