Fig. 5: LACV is retained in the cis-Golgi in muscle cells.
From: Skin muscle is the initial site of viral replication for arboviral bunyavirus infection
Neural stem cells (NSCs) and SMEVs were infected with LACV and released virus was quantified in the supernatant by plaque assay (A; n = 6 biological replicates per time point for each sample type). Golgi staining (GM130; green) was performed in infected SMEVs (5 dpi) and NSCs (1 dpi) (B) and LACV (magenta) occupying the cis-Golgi was quantified as a proportion of virus contained within the cis-Golgi compartment demarcated by GM130 compared to total LACV within individual cells (C). Peritoneal muscle from mice infected IP in vivo was removed and stained for LACV (D, E; magenta) and myosin (D; white) or GM130 (E; green). The 3D volumes of images from (B, D, and E) were rendered for visualization as partially transparent overlays (GM130, myosin) or fully opaque structures (LACV). For (C), 2 SMEV tissues (n = 42 cells) and 4 replicate NSC wells (n = 50 cells) were quantified. For (D, E), images are representative of peritoneal muscle from 2 mice. A, C Data plotted as means with error bars representing SD. ****p < 0.0001 (p = 2.15 × 10−12) by unpaired, two-tailed Student’s t test. Scale bars represent (B) 5 μm, (D) 20 μm and (E) 10 μm. Source data are provided as a Source Data file.
