Fig. 2: Ectopic prostate tumour development triggers changes in the fecal microbiota in independent syngeneic mouse models.

a Growth phases analysis of TRAMP-C2 tumour cells injected into the flank of immunocompetent single-housed C57BL/6 N mice (n = 12/group). b Relative abundance of bacterial phyla of fecal samples harvested from tumour-free animals (baseline) and three time points corresponding to minimal masses (first detectable tumour), actively growing and late stage (end point) TRAMP-C2 tumours (n = 8/group). p = 8e⁻⁴, two-sided Welch’s t-test was used for comparing groups. c Shannon diversity index at family taxonomic level of 16S rRNA sequences associated with fecal samples harvested from single-housed C57BL/6 N mice injected with TRAMP-C2 prostate tumour at different development stages (n = 8/group). p = 3e⁻⁴, p = 2e⁻⁴, for active growth and end point, respectively, two-sided Welch’s t-test was used for comparing groups. d Bray-Curtis beta diversity analysis and PCA visualization of 16S rRNA sequences associated with fecal samples from mice injected with TRAMP-C2 prostate cancer cells and at different phases of tumour development (n = 8/group). e Tumour volume analysis at the 4 week time-point of immunocompetent C57BL/6 J mice injected with syngeneic Pten^−/− or Pten^−/−; Rb1^−/− prostate tumour cells or control animals without tumour cells (n = 8/group). p = 6e⁻⁴, two-sided Welch’s t-test was used for comparing groups. f Relative abundance of bacterial phyla of fecal samples harvested from tumour-free animals (no tumour) and mice bearing Pten^−/− or Pten^−/−; Rb1^−/− prostate tumours (n = 8/group) 4 weeks after tumour cell injections. p = 0.01 for firmicutes and p = 0.001 for actinobacteria, two-sided Welch’s t-test was used for comparing groups. g Shannon diversity index at family taxonomic level of 16S rRNA sequences associated with fecal samples from single-housed tumour-free C57BL/6 J animals (no tumour) and animals with either Pten^−/− or Pten^−/−; Rb1^−/− prostate tumours (n = 8/group). p = 0.02, two-sided Welch’s t-test was used for comparing groups. h Bray-Curtis beta diversity analysis and PCA visualization of 16S DNA sequences associated with fecal samples from mice injected with Pten^−/− and Pten^−/−; Rb1^−/− prostate tumour cells and different tumour burden (n = 8/group). Graphs are mean ± SEM.