Fig. 3: Single cell RNA-sequencing identifies a cytotoxic signature of triple-integrin-expressing peripheral blood T cells in CD. | Nature Communications

Fig. 3: Single cell RNA-sequencing identifies a cytotoxic signature of triple-integrin-expressing peripheral blood T cells in CD.

From: Etrolizumab-s fails to control E-Cadherin-dependent co-stimulation of highly activated cytotoxic T cells

Fig. 3

A UMAP plot showing unsupervised clustering of sorted α4+ memory T cells (Tmem) from the peripheral blood of a donor with CD and a non-IBD control (CON) based on Leiden algorithm at resolution 0.5. B UMAP plot showing the distribution of cells from CON (gray) and CD (light green). Pie chart illustrates the overall proportions. C UMAP plot showing the distribution of cell that do not express ITGB7 (ITGB7neg, tawny), express ITGB7, but not ITGB1 (ITGB7sp, magenta) or express ITGB7 and ITGB1 (ITGB1/7dp, dark green). D Pie charts illustrating the proportions of ITGB7neg, ITGB7sp, ITGB1/7dp cells per Leiden cluster. E Heatmap showing the fraction of cells expressing and the mean expression of the indicated genes. F UMAP plots showing the distribution of cells expressing GNLY (left) and GZMB (right). G Heatmap showing the fraction of cells expressing and the mean expression of the indicated genes in ITGB1/7dp and ITGB7sp cells. H Representative and quantitative flow cytometry of granulysin (GNLY, left) and granzyme B (GZMB, right) protein expression on peripheral blood CD3+CD45RA−α4+β7+ memory T cells, stratified according to their β1 expression, from CON and CD (n = 6–10). Significant outliers were detected by 1% ROUT test and removed from analysis. Two-way ANOVAs with Sidak’s multiple comparisons test were performed. Source data are provided as a Source Data file.

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