Fig. 4: β7⁺ memory T cells (Tmem) that evade blockade by vedolizumab (VDZ) readily induce CD103.

Representative and quantitative flow cytometry of CD103 expression on sorted β7⁺ and β7⁻ CD4⁺ Tmem (CD3⁺TCRVα7.2⁻CD4⁺CD8a⁻CD45RA⁻β7^+/−) (A, B) and on sorted β7⁺ and β7⁻ CD8⁺ Tmem (CD3⁺TCRVα7.2⁻CD4⁻CD8a⁺CD45RA⁻β7^+/−) (C, D) after 3 days of incubation with anti-CD2/3/28 stimulation beads in the presence or absence (w/o) of 10 ng/ml TGFβ1. n = 5. E–H Representative and quantitative flow cytometry of CD103 expression on sorted β7⁺ CD4⁺ Tmem (CD3⁺TCRVα7.2⁻CD4⁺CD8a⁻CD45RA⁻β7⁺) (E, F) and on sorted β7⁺ CD8⁺ Tmem (CD3⁺TCRVα7.2⁻CD4⁻CD8a⁺CD45RA⁻β7⁺) (G, H) treated with or without (untreated) 10 µg/ml VDZ before transmigration over a MAdCAM-1- and VCAM-1-coated membrane and subsequent incubation with anti-CD2/3/28 stimulation beads in the presence or absence of 10 ng/ml TGFβ1. n = 5. Significant outliers were detected by 1% ROUT test and removed from analysis. Two-way ANOVAs with Tukey’s multiple comparisons test were performed. Data are displayed as bar plots depicting mean ± SEM. Source data are provided as a Source Data file.