Fig. 2: Proper regulation of jasmonic acid responses and programmed cell death at the petal base control the timing of petal abscission.
From: Petal abscission is promoted by jasmonic acid-induced autophagy at Arabidopsis petal bases
a Profile view of mock- and jasmonic acid (JA)-treated WT, ag and dad1 inflorescences. Opened flowers with petals are indicated by gray asterisks. Scale bar = 1 cm. b Quantification of abscission timing, shown as individual data points (left) and violin plots (right) for each genotype (WT: n = 14; WT + JA: n = 22; ag: n = 19; ag + JA: n = 26; dad1: n = 16; dad1 + JA: n = 27). Different letters indicate statistically significant differences based on one-way ANOVA test (p = 1.1 ×10−16) and post-hoc Tukey’s HSD test (p < 0.05: See the Data Source file for all combinations of the exact p-values). c DAB staining of mock- and JA-treated WT, ag and dad1 petals at position +3 flowers. Scale bar = 100 µm. d Quantification of DAB-stained area (µm2), shown as individual data points (left) and violin plots (right) for each genotype. Black dots and vertical lines indicate mean and SD, respectively (WT: n = 36; WT + JA: n = 34; ag: n = 21; ag + JA: n = 12; dad1: n = 22; dad1 + JA: n = 40). Different letters indicate statistically significant differences based on one-way ANOVA test (p = 2.3 ×10−10) and post-hoc Tukey’s HSD test (p < 0.05: See the Data Source file for all combinations of the exact p-values). e Trypan blue staining of mock- and JA-treated WT, ag and dad1 petals from position +3 flowers. Scale bar = 100 µm. f DAD1pro:GUS staining pattern in WT petals from position –3 (left), –1 (middle), and +1 (right) flowers. Scale bar = 500 µm. gDAD1–GFP accumulation in WT stamens (g) and petals (h) at position –3 (left), –1 (middle), and +1 (right) flowers. Magenta: cell wall; green: DAD1–GFP. Scale bar = 50 µm. i–k JAZ9–VENUS accumulation in mock- (left and middle) and JA-treated (right) WT, ag, and dad1 petals from position –3 (left) and 0 (middle and right) flowers. l–n BFN1pro:nGFP expression in mock- (left and middle) and JA-treated (right) WT, ag and dad1 petals from position –3 (left) and 0 (middle and right) flowers. Magenta: cell wall; green: JAZ9–VENUS or GFP. Scale bars = 50 µm.
