Fig. 6: Genome-wide identification of ANAC102-regulated targets during petal abscission.

a gANAC102–GFP accumulation in petal bases from position +3 flowers. Scale bars = 100 µm. Top, merge; middle, GFP; bottom, propidium iodide (PI). b ANAC102 input and ChIP data. Left, Input; Right, ChIP. Upper panels show the average profile around the detected peak. Lower panels show read density heatmaps around each detected peak. c ANAC102 cis-motifs identified by de novo motif analysis under each ANAC102 peak summit. ANAC047 and NAM belong to the SNAC family, as does ANAC102. p-values obtained from HOMER are shown. d Venn diagram showing the overlap between direct ANAC102 targets (purple), down-regulated genes in the snac mutant (green), and down-regulated genes in the dad1 mutant (yellow). e GO-term enrichment analysis using ANAC102-bound genes and down-regulated genes in the snac mutant. Selected GO terms related to stress response, plant hormones, transcription and cellular process are shown with their –Log₁₀ adjusted p-value. The p-values for GO analysis are obtained by converting the Z-scores based on a two-tailed Z-test. f Interactive graph view of GO terms generated by REVIGO. The node radius indicates the specificity, and the color shading corresponds to the p-values. The p-values for GO analysis are obtained by converting the Z-scores based on a two-tailed Z-test. Highly similar GO terms are linked by edges, with edge thickness indicating the degree of similarity. g Hierarchical clustering of 581 genes during petal abscission. The heatmap shows the Log₁₀ fold-change of differentially expressed genes based on the transcriptome data in d.