Fig. 8: Local changes in autophagic activity affect the timing of petal abscission.
From: Petal abscission is promoted by jasmonic acid-induced autophagy at Arabidopsis petal bases
a Spatiotemporal observation of VENUS in petal bases of atg7-2 (left) and atg7-2 ANAC102pro:mVENUS–ATG7 (right) by confocal microscopy. Scale bars = 100 µm. b Higher magnification images of VENUS in petal bases of atg7-2 ANAC102pro:mVENUS–ATG7 by confocal microscopy. Image in right is magnified image of the boxed region in left. Scale bars = 100 µm. c Side view of atg7-2 and atg7-2 ANAC102pro:mVENUS–ATG7 inflorescences. Open flowers with petals are indicated by asterisks. Scale bar = 1 cm. d Quantification of the timing of abscission, shown as individual data points (left) and violin plots (right) for each genotype. Black dots and vertical lines indicate mean and SD, respectively. n = 10. One-tailed Student’s t test, *p = 9.1 × 10−3. e DAB staining of atg7-2 and atg7-2 ANAC102pro:mVENUS–ATG7 petals of position +3 flowers. Scale bars = 100 µm. f Trypan blue staining of atg7-2 and atg7-2 ANAC102pro:mVENUS–ATG7 petals of position +3 flowers. Scale bar = 100 µm. g Spatiotemporal observation of GFP in petal bases of atg5-1 gATG5–GFP (left) and atg5-1 gATG5–GFP ANAC102pro:amiRATG5 (right) by confocal microscopy. Scale bars = 100 µm. h Higher magnification images of GFP in petal bases of atg5-1 gATG5–GFP by confocal microscopy. Image in right is magnified image of the boxed region in left. i Side view of WT (left) and ANAC102pro:amiRATG5 (right) inflorescences. Open flowers with petals are indicated by asterisks. Scale bar = 1 cm. j Quantification of the timing of abscission, shown as individual data points (left) and violin plots (right) for each genotype. Black dots and vertical lines indicate mean and SD, respectively. n = 10. One-tailed Student’s t test, *p = 0.02. k DAB staining of WT (left) and ANAC102pro:amiRATG5 (right) petals of position +3 flowers. Scale bars = 100 µm. l Trypan blue staining of WT (left) and ANAC102pro:amiRATG5 (right) petals of position +3 flowers. Scale bar = 100 µm.
