Fig. 5: Locus-specific epigenetic editing to fine map effector genes in rs10815466 risk locus. | Nature Communications

Fig. 5: Locus-specific epigenetic editing to fine map effector genes in rs10815466 risk locus.

From: Integrating leiomyoma genetics, epigenomics, and single-cell transcriptomics reveals causal genetic variants, genes, and cell types

Fig. 5

a The dot plot shows the significance of SNPs in the rs584154480 lead SNP risk locus. ChIP-Seq tracks show the signal intensity of H3K27ac and arc plots show the intensity of Hi-C measured chromatin contact frequency in the same genomic region for UF tissue and MyoF patient samples. b The Bar plot shows ChIP-qPCR measured H3K27ac levels at the indicated region on Day 0 and 15 days after the induction of dCas9-KRAB. c RNA-Seq bigwig tracks show relative expression of KANK1 locus before and after 15 days of dCas9-KRAB recruitment to the KANK1 target locus with sgRNA-1. Tracks heights are normalized to read depth. d The bar plot shows mRNA levels of indicated genes in dCas9-KRAB expressing smooth muscle cells with control sgRNA and sgRNA targeting around the rs10815466 lead SNP. The relative mRNA levels of all nine genes proximal or 3D linked to the rs10815466 lead SNP risk loci were measured by RT-qPCR. Two-tailed student t-tests were used for all statistical comparisons. Error bars in Fig. 5b, d indicate the standard error of the mean of three independent biological replicates (n = 3).

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