Fig. 3: Benchmark of µPAC column designs and gradient lengths. | Nature Communications

Fig. 3: Benchmark of µPAC column designs and gradient lengths.

From: Micropillar arrays, wide window acquisition and AI-based data analysis improve comprehensiveness in multiple proteomic applications

Fig. 3

A Bars indicate average number of identified proteins at 1% FDR on protein level when acquiring using different column lengths, gradient times and input amounts indicated of a 8:1:1 H:Y:E proteome mix. Error bars indicate standard deviations, n = 2 technical replicates for 120 min 10 ng and 50 ng n = 3 technical replicates for all other conditions. Bars indicate means per condition. B Comparison of all columns for 30 min gradient from 10–400 ng peptide load. C Comparison of all columns for 60 min gradient from 10–400 ng peptide load. Data for (B) and (C) is already presented in panel A but displayed differently for easier visual comparison. Source data are provided as a Source Data file.

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