Table 1 Experimental overview to assess the advanced platform performance in part and overall

From: Micropillar arrays, wide window acquisition and AI-based data analysis improve comprehensiveness in multiple proteomic applications

Platform Constituent Assessed

Question

Display item

LC setup

Packed columns vs μPAC

Fig. 2

Comparison of different μPACs

Fig. 3

Protein IDs/min and throughput

Fig. 4 and Table 2

MS acquisition

WWA vs DDA

Fig. 5A, B

Isolation width evaluation for WWA

Fig. 5D, E

Search engine

CHIMERYS vs classical search engine

Fig. 5A–C

Entrapment experiment for FDR estimation

Fig. 5C

Application – low input & single cells

Influence of WWA + /− MBR on protein IDs and quantification for (very) low inputs

Fig. 6A–D

Application - two-species proteome mix

Evaluation of quantitative accuracy for DDA and WWA using CHIMERYS

Supplementary Fig. 6

Application – AP-MS – entire workflow

Impact of entire workflow (μPAC, WWA, CHIMERYS) on identification of additional interaction partners in AP-MS

Fig. 7 and Table 3

Back to article page