Fig. 4: Sera from mice immunized with S2 immunogens can neutralize a broad range of rVSV-CoVs.

a-e Pre-titrated amounts of rVSV-CoVs were incubated with serial 3-fold dilutions of sera from mice (n = 10/immunized group) immunized with respective S antigens or PBS at RT for 1 hr. Virus-sera mixtures were then added to monolayers of Vero cells. At 10 hr post-infection, cells were fixed, and nuclei were counterstained. Infected cells were scored by BioTek Cytation5 for presence of GFP. Sera from 10 mice are included in each group. Area under the curve (AUC) was calculated from normalized infectivity levels. One-way ANOVA with Tukey’s multiple comparisons test (a, c, e) or Welch’s ANOVA with Dunnett’s T3 multiple comparisons test (b, d) were run based on normality and homoscedasticity. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05 a PBS:HexaPro, HexaPro-SS, HexaPro-S2, HexaPro-SS-PentaStalks, Hexapro-SS-Δstalk p = <1e-15, 2.86e-9, 2.61e-7, 1.48e-11, 1.11e-11, HexaPro:HexaPro-SS, HexaPro-S2, HexaPro-SS-PentaStalks, Hexapro-SS-Δstalk p = 5.64e-10, 3.01e-11, 1.04e-7, 1.34e-7; b PBS:HexaPro-SS-PentaStalks, Hexapro-SS-Δstalk p = 7.37e-4, 2.40e-3; c PBS:HexaPro, HexaPro-SS, HexaPro-S2, HexaPro-SS-PentaStalks, Hexapro-SS-Δstalk p = 5.14e-3, 2.75e-6, 9.77e-7, 6.54e-6, 6.68e-7; d PBS:HexaPro, HexaPro-SS, HexaPro-S2 p = 6.20e-3, 1.31e-2, 7.20e-3. Source data are provided as a Source Data file.