Fig. 3: Spatial response profiles measured from V1 in the right hemisphere of a head-fixed macaque in one GCaMP imaging experiment.
From: Mesoscopic calcium imaging in a head-unrestrained male non-human primate using a lensless microscope
a Illustration of experimental setups for capturing ground truth (left) and Bio-FlatScopeNHP imaging (right). Small gratings at 6 different positions are used as visual stimuli in the experiments. b Average time course of GCaMP response to the flashed gratings over the center 2 mm × 2 mm area of the FOV from ground truth (left) and Bio-FlatScopeNHP reconstructions (right). ∆F/F indicates the relative changes in fluorescence. Shaded area ± SEM. c Visual stimulus coordinates in degrees of visual angle. d An example image captured by ground truth widefield microscope (left). Scale bar, 500 μm. Spatial distribution of response amplitudes for different stimulus positions fitted by a Gaussian function in ground truth captures (right). The data points shown in the right panel represent the mean response measured within the cortical regions highlighted by color coding in the left panel. The main response from blank trials is subtracted correspondingly. e An example image captured and reconstructed using Bio-FlatScopeNHP (left) and fitted spatial distribution (right) from the reconstructions. f Response amplitude of ground truth and Bio-FlatScopeNHP at different stimulus conditions. Scale bar, 500 μm. g Peak position comparison between ground truth captures and Bio-FlatScopeNHP reconstructions. Source data are provided as a Source Data file.
