Fig. 4: High-throughput single-cell RNA-seq of NK cells reveals gp96–/– clusters with distinct transcriptional signatures. | Nature Communications

Fig. 4: High-throughput single-cell RNA-seq of NK cells reveals gp96–/– clusters with distinct transcriptional signatures.

From: Heat shock protein gp96 drives natural killer cell maturation and anti-tumor immunity by counteracting Trim28 to stabilize Eomes

Fig. 4

A A t-Distributed Stochastic Neighbor Embedding (tSNE) and graph visualization of the 15699 single NK cells defining 6 clusters. B Heatmap of marker genes in scRNA-seq clusters. Columns: single cells. Rows: cluster marker genes. Representative genes that are differentially expressed are on the left. C Cell number of Ncr1Cregp96fl/fl and WT NK cells within each cluster. D Feature dot plot showing the relative expression levels of the indicated genes in each cluster from (A). E Velocity analysis of the origin and direction of NK cell maturation. Velocity fields were projected onto the t-SNE plot. F KEGG analysis of DEGs for indicated clusters. Statistical significance was determined using one-sided Fisher’s Exact Test with adjustments for multiple comparisons typically using methods like the Benjamini-Hochberg procedure. Selected KEGG terms with adjusted P values < 0.05 are shown.

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