Fig. 5: Effect of gp96 on Eomes expression in NK cells.

A The fold changes indicated the difference in relative transcript expression of Eomes-bound genes in DX5⁺ splenic NK cells between WT compared with Ncr1^Cregp96^fl/fl mice, as determined by RNA-seq. Y-axis showed Log2 fold changes of WT vs. KO mice. B Correlation analysis of Log₂ fold changes of Eomes-targeted genes between gp96^–/– NK versus WT NK and Eomes^–/– versus WT NK. C Flow cytometry analysis of levels of Eomes between Ncr1^Cregp96^fl/fl and WT NK cells. D Western blot analysis of Eomes levels between Ncr1^Cregp96^fl/fl and WT NK cells from spleen and bone marrow (BM). E Flow cytometry analysis of DX5⁺ NK cell percentage in WT or gp96 KO BM hematopoietic stem cells infected with Eomes or control Lentiviral vector. Mean ± SD of three replicates is shown. Statistical significance was determined using two-tailed unpaired t test. *p <  0.05, ***p <  0.001, ****p <  0.0001. p values: (B) p = 0.0004, (C) p <  0.0001 (spleen), p =  0.0002 (BM), (D) p <  0.0001 (spleen), p <  0.0001 (BM), (E) p =  0.0003 (WT-control vs KO-control), p =  0.0117 (KO-control vs KO-Eomes).