Fig. 1: ART Individuals carry a different mitochondrial DNA genotype than their spontaneously conceived peers. | Nature Communications

Fig. 1: ART Individuals carry a different mitochondrial DNA genotype than their spontaneously conceived peers.

From: Children born after assisted reproduction more commonly carry a mitochondrial genotype associating with low birthweight

Fig. 1

a Schematic overview of the mtDNA indicating in orange the regions that were excluded from the analysis. Adapted with permission from ref. 112, Copyright © 2009 International Union of Biochemistry and Molecular Biology, Inc. b, c Fisher’s exact test shows no significant differences in the prevalence of the different haplogroups (b) and subhaplogroups (c) across ART and SC individuals. d Fisher’s exact test shows no significant differences in the prevalence of ART and SC individuals with homoplasmic variants located in the different categories. Percentages do not add up to 100% because one individual can have more than one variant. e Overview of all heteroplasmic variants identified. Four of the tRNA genes were not represented in our dataset. Every bubble represents one variant and the size of the bubble is proportional to the variant’s heteroplasmic load. f, g Sum of the heteroplasmic loads identified per sample, categorized according to the tissue of origin (f) and age at sampling (g). Kruskal–Wallis Test shows no significant differences. Blood n = 168, Placenta n = 117, Saliva and Buccal Swab n = 166, at birth n = 163, 9 years old n = 106, 18 years old n = 182. h Number of variants identified per individual. Chi-square test shows no significant differences (non-synonymous variants, p = 0.067). i Mutation rate per region or protein-coding gene, per individual in ART and SC. j Sum of loads of the heteroplasmic variants found per individual and per location. Mann–Whitney U Test shows no significant differences. n = 270 ART, n = 181 SC. k Component matrix generated by the factor analysis. l Hypercube representing the covariance between the different categories of heteroplasmic variants. The colours indicate categories that co-variate in the same factor. m ART individuals more frequently carry higher scores for factor 2 (*Mann–Whitney U Test, p = 0.021). n = 270 ART, n = 181 SC. Horizontal bars in panels (f), (g), (j) and (m) represent the mean of the values in the scatter plot, each dot represents a sample. Source data are provided as a Source data file.

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