Fig. 5: γδTCR3-4 recognizes multiple Merkel cancer cell lines.

A The clonally expanded γδ TCR from patient E-013 was cloned and expressed into Jurkat76 cells (γδTCR3-4) along with mis-paired control cells (γδTCR1-4 and γδTCR3-5). Transduced γδTCR Jurkat76 cells were screened for reactivity against PBMCs and various cell lines including myeloma H929, leukemic cell lines THP1 and K562, human umbilical vein endothelial cells (HUVEC), ovarian cancer cell lines OVCAR3 and CaOV3, melanoma cell lines A375, 888mel and 624mel and Merkel cancer cell lines MS-1, MCC14-2 and MCC26. Fold change in CD69 expression was normalized by dividing the co-culture condition by the staining from Jurkat76 cells alone condition. MCC lines were denoted as negative (-) or positive (+) for Merkel cell polyomavirus (MCPyV). B Example histogram of CD69 expression on Jurkat76 γδTCR3-4 after co-culture with MCC26 at 1:2 target to effector ratio. C Jurkat76 cells were co-cultured with increasing numbers of MCC target cells. Non-transduced Jurkat76 cells are in blue, mis-paired γδ TCRs are in open circles and open squares, and γδTCR3-4 is in red circles. D Jurkat76 cells were co-cultured with increasing number of PBMCs, H929 myeloma, HUVEC and 888mel and 624mel melanoma cells. C, D Co-culture experiments were plated in duplicate and performed in two independent experiments. Data are presented as mean values +/- SD. Source data for (A, C and D) provided as a source data file.