Fig. 3: Cell culture. | Nature Communications

Fig. 3: Cell culture.

From: Mechanically resilient hybrid aerogels containing fibers of dual-scale sizes and knotty networks for tissue regeneration

Fig. 3

a, b CLSM images showing 3D and orthogonal view of GFP-labeled dermal fibroblasts in the aerogels after seeding for 3 and 7 days. c CLSM images showing human neural progenitor/stem cells cultured on NFA, MFA, and NF/MF-A1 samples for 7 days. Neurons were stained with β-III tubulin in green. 3D view and depth analysis reveal the distribution of neurons/neurites on NFA, MFA, and NF/MF-A1 after 7 days of culture. d Schematic illustrating the migration of cells from bottom to top. The schematic was created in BioRender.com. e CLSM images showing GFP-labeled dermal fibroblasts on the bottom surface and top surface of NF/MF-A1 aerogels at day 0, 3, 6, and 9. Scale bar = 100 µm for (a–c); 1 mm for (e). CLSM imaging was repeated three times and similar results were obtained. NFA nanofiber aerogels coated with 1% gelatin, MFA microfiber aerogels coated with 1% gelatin, NF/MF-A1 hybrid aerogels containing NF/MF at a ratio of 50:50 (w/w) coated with 1% gelatin.

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