Fig. 4: Nuclear export signal and ribosomal biogenesis promote prostate cancer progression and aggressiveness. | Nature Communications

Fig. 4: Nuclear export signal and ribosomal biogenesis promote prostate cancer progression and aggressiveness.

From: Androgen deprivation induces double-null prostate cancer via aberrant nuclear export and ribosomal biogenesis through HGF and Wnt activation

Fig. 4

a Individual UMAP visualization of cells from DoubleTg (top left, gray) and TripleTg (top middle, dark blue) prostates, and integrated cells colored by cell type identities (bottom). UMAP plot of epithelial cells re-clustered and labeled as BE, basal epithelial cells; LE, luminal epithelial cells; UrLE, urethral epithelial cells; OE, other epithelial cells (top right). b UMAP plots showing indicated gene expression, separated by each genotype. Color intensity indicates the scaled expression level. c GSEA compares hMETtg+ cells from TripleTg versus DoubleTg mice. See Supplementary Data 3. d Violin plots visualizing the indicated gene expressions in hMETtg+ cells (DoubleTg, n = 4417; TripleTg, n = 2144) of each genotype. e qPCR analysis of the indicated genes shown as fold changes in the indicated tissues from three biological replicates. f Heatmap depicting average expression of genes associated with indicated pathways in each cluster. g Pseudotime trajectory plots of hMETtg+ cells (n = 2144) from TripleTg mice, visualized on UMAP plots by pseudotime (top) and cluster identity (bottom). h Linear pseudotime expression plots showing dynamics of indicated gene expression over pseudotime in hMETtg+ cells from TripleTg mice. Dots correspond to individual cells colored by cluster identity. i Violin plot visualizing the expression levels of indicated genes from TripleTg mice (LE1, 5–9, n = 1934; LE2, n = 1165; LE3, n = 1666; LE4, n = 1096). j Bubble charts showing GSEA by comparing LE2, LE3, or LE4 versus other LEs in TripleTg sample. Color and size of bubbles represents NES and weighted numbers of genes. See Supplementary Data 4–6. k Representative images of IHC using indicated antibodies on adjacent sections from TripleTg mice. Representative images with consistent results from three biological replicates are shown. Scale bars, 25 μm. In d–i, data are mean ± s.d. **P < 0.01 and ***P < 0.001, two-tailed Wilcoxon Rank Sum tests (d, i), unpaired two-tailed t-tests (e). Green or blue bar indicates the mean value (d, i). Source data and the exact P values are provided in the Source Data file.

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