Fig. 3: Overexpression of antisense-TRI5 inhibits DON biosynthesis.
a Schematic drawing of the TRI5 ORF, its sense or antisense transcripts, and lncRNA RNA5P as well as the PRP27-antiTRI5 construct in which a 299-bp fragment was cloned between the RP27 promoter (RP27) and CaMV ployA signal terminator (T). The black box marks the intron of TRI5. Arrows indicate the position and orientation of the primers. The fragment was amplified with the primer pair SF1/SR2 for the generation of the PRP27-antiTRI5 construct, and the primer pairs TF1/TR2 and AF3/AF4 were used to assay the expression of TRI5 and antisense-TRI5, respectively. b Relative expression levels of antisense-TRI5 and TRI5 in 3-day-old LTB cultures of the wild type (PH-1) and PRP27-antiTRI5 transformant (RAT1 and RAT2). The relative expression level in PH-1 was arbitrarily set to 1. c DON production in 7-day-old LTB cultures of PH-1 and PRP27-antiTRI5 (RAT1 and RAT2). d Wheat heads inoculated with the indicated strains were examined for head blight symptoms at 14 days post-inoculation (dpi). Black dots mark the inoculated spikelets. For b and c, mean and standard deviation were estimated with data from three (n = 3) independent replicates (marked with black dots on the bars). For DON production, different letters indicate significant differences based on the one-way ANOVA followed by Turkey’s multiple range test. Differences were considered statistically significant when p-value is <0.05. The exact p-values are shown in the Source Data file.
