Fig. 3: Mutation analysis of the interactions SAP05 with TFs and RPN10. | Nature Communications

Fig. 3: Mutation analysis of the interactions SAP05 with TFs and RPN10.

From: Molecular basis of SAP05-mediated ubiquitin-independent proteasomal degradation of transcription factors

Fig. 3

a ITC fitting curves of wild-type and mutant OY-M SAP05 titrated to wild-type AtSPL5. NB, no apparent binding under our experimental conditions. b ITC fitting curves of wild-type OY-M SAP05 titrated to wild-type and mutant AtSPL5. c GST pull-down assay using GST-tagged OY-M SAP05 to pull down wild-type and mutant AtSPL5. Source data are provided as a Source Data file. Representative images, n = 3. d ITC fitting curves of wild-type and mutant OY-M SAP05 titrated to wild-type AtGATA18. e ITC fitting curves of wild-type OY-M SAP05 titrated to wild-type and mutant AtGATA18. f GST pull-down assay using GST-tagged wild-type and mutant AtRPN10 to pull down co-purified wild-type OY-M SAP05. Source data are provided as a Source Data file. Representative images, n = 3.

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