Fig. 4: Mutagenesis analysis of SAP05-mediated degradation of TFs. | Nature Communications

Fig. 4: Mutagenesis analysis of SAP05-mediated degradation of TFs.

From: Molecular basis of SAP05-mediated ubiquitin-independent proteasomal degradation of transcription factors

Fig. 4

a Western blot analysis of OY-M SAP05-mediated degradation of AtSPL5 using purified human 26 S proteasomes in the presence of wild-type AtSPL5, OY-M SAP05 and AtvWA (AtRPN10 vWA domain). b–e Western blot analysis of the degradation of AtSPL5 in the presence of OY-M SAP05 mutants (D106A or W85A) or AtSPL5 mutants (Q105A or R121A). Source data are provided as a Source Data file. Representative images, n = 3. f Quantification of the percentage of retained GST-SPL5 in the degradation assay, corresponding to (a–e) (Mean ± S.E.M.; n = 3 independent experiments).

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