Fig. 1: Dimeric cryo-EM structure of Drp1.

a Previously identified domains in human Drp1 are indicated (GTPase, Bundling Signaling Element (BSE), Stalk, and Disordered Region). The corresponding residue numbers represent the architecture of Drp1 isoform 1 (Drp1-1). The DSP domains are labeled in gray. b Density of the dimeric cryo-EM dimer of Drp1 with a low pass filter of 6 Å applied (orange = chain A, yellow = chain B) is shown. c The docked chain structures within cryo-EM density after refinement are presented as ribbon diagrams. d Chain A (chA) of solution structure highlights the relative positions of domains in this state. The GTPase domain (green), BSE (red) and stalk region (blue) are indicated. The variable domain (VD, yellow dashed line) is not shown as this was not resolved. e Top down and side views of the Drp1 dimer solution structure are presented with corresponding dimensions.