Fig. 3: Isotype and specificity of neutralizing antibodies.

IgG was purified from serum pools of CTH522/CAF®01 vaccinated B6C3F1 mice (n = 100) and control mice (n = 100). a Neutralization of C.t. SvD using purified IgG were compared to the unfractionated serum samples. Each dot represents the mean neutralization of duplicate readings and results are presented from two individual experiments (serum and IgG from Exp.1 and Exp.2, Fig. 5). b Correlation of VD4_6-22^D/E specific IgG at a serum dilution of 1:21870 (n = 66) and the NT₅₀ (n = 66) was analyzed using Two-tailed non-parametric Spearman’s rank correlation coefficient, r = 0.8641, 95% CI [0.7838, 0.9160]. c CTH522, CTH523, CTH518 (extVD4^D/E/F/G), and a VD4_6-22^D/E/F/G construct were incubated with CTH522 specific mouse serum and control serum each diluted 1:200, before being mixed with an equal volume of C.t. SvD, E, F, and G and inoculated onto HaK cells. Data points represent the calculated mean NT₅₀ of triplicate readings and each bar shows the mean with SEM of three individual determinations. a–c Source data are provided as a Source Data file.