Fig. 4: In vitro and in vivo neutralization.
a Experimental overview of in vitro and in vivo neutralization assays. b In vitro neutralization: Pools of B6C3F1 mouse and human CTH522 specific serum and control serum were heat inactivated, titrated and mixed with a fixed concentration of C.t. SvD, inoculated onto a HaK cell monolayer in duplicates and the NT50 were calculated. Each bar represents mean NT50 with SEM of three individual determinations. c In vivo neutralization. Serum pools were diluted 1:2 or 1:4 with C.t. SvD and inoculated i.vag. into depo-provera treated C3H/HeN mice (n = 10/group). IFU were determined in swab samples taken at PID3 and 7. Data points represent individual log10 IFU per mouse and line represents median IFU levels with 25th and 75th percentiles. Two-tailed Mann–Whitney U test was used for comparison among groups. d Comparison of C.t. SvD, E, F, and G NT50 using human CTH522 specific serum in the standard HaK cell line and a human endocervical cell line (End1/E6E7). Only serum samples (n = 7) giving a SvD NT50 > 200 in the standard assay were evaluated using the End1/E6E7 cell line. Data points represent NT50 calculated from mean of duplicate or triplicate readings. a Figure and c, d Mouse and human symbols were created with Biorender.com. b–d Source data are provided as a Source Data file.
