Fig. 5: The role of CTH522 specific antibodies during C.t. infection.

a Overview of the experimental outline and treatments. Groups of C3H/HeN mice were CD4⁺/CD8⁺ depleted before and during an i.vag. C.t. SvD infection. A non-depleted group was included as control. At PID-3, depleted mice received either purified IgG from control or CTH522 vaccinated B6C3F1 mice. In Exp. 2 mice received an additional dose at PID15. b Kinetics of clearance in CD4⁺/CD8⁺-depleted mice receiving IgG from control mice (PID 3 and 7 n = 29, PID10 n = 28, PID14-24 n = 27) compared to non-depleted mice (n = 30). Levels of IFU were determined in vaginal swab samples at several time points post infection and compared to levels obtained in non-depleted mice. Results are presented as median Log10 IFU/mouse with 25th and 75th percentiles. c Kinetics of C.t. SvD clearance of CD4⁺/CD8⁺ depleted mice receiving IgG purified from either CTH522 vaccinated or control mice. Two individual experiments (Exp. 1 and Exp. 2) are presented. Results are presented as Log10 IFU/mouse and presented as a violin plot with median and 25th and 75th percentiles, all data points. The number of mice in each group are shown in the violin plot. Mixed-effect model with the Geisser-Greenhouse correction for repeated measures were used for comparison among groups. d Levels of IFU in homogenates of uGT (oviducts/ovaries) at PID29 in the two individual experiments (Exp. 1 n = 4, Exp. 2 n = 16). The data are presented as a violin plot plots with median and 25th and 75th percentiles, all data points. Dotted line indicates the 1000 IFU level e Histological sections of 6 oviducts (5x magnification) from mice with different C.t. clearance kinetics (Panel 1–6) stained with an in-house rabbit anti-MOMP antibody visualizing the C.t. inclusions. The oviduct lumens are denoted with asterisks and C.t. inclusions with arrows. The presented oviduct is representative for the mouse examined and only performed on a total of 7 mice in Exp. 1. b–e Source data are provided as a Source Data file.