Fig. 7: Longevity of CTH522 specific immunity and protection. | Nature Communications

Fig. 7: Longevity of CTH522 specific immunity and protection.

From: Immune signature of Chlamydia vaccine CTH522/CAF®01 translates from mouse-to-human and induces durable protection in mice

Fig. 7

a Experimental outline. B6C3F1 mice were immunized with 10 µg CTH522/CAF®01 or Sham-immunized. b Kinetics of CTH522 specific IgG: week 8, 31, 56 n = 20 (except CTH522 week 56 n = 18), week 59 and 61, n = 6, NT50: CTH522 week 8, 31, 56 n = 19 (except week 56 n = 17), Controls n = 20, week 59 and 61, n = 6 and IFN-γ responses: n = 6 (except Controls week 61 n = 4). Dotted vertical line indicates time of challenge. c Six (n = 9) and 56 (n = 6) weeks post 1st vaccination, splenocytes were stimulated with CTH522. Data points represent the percentages of cytokine positive (IFN-γ, IL-2, IL-17A, TNF-α) CD44highCD4+ T cells out of all CD4+ T cells. d Data points (n = 6) represent the percentage of IL-17A− (Th1) and IL-17A+ (Th17) out of all CD4+ T cells producing the cytokine TNF-α, IL-2, and IFN-γ in any combination. e, f 58 weeks post 1st vaccination mice were challenged i.vag. with C.t. SvD and IFU recovered from swabs at PID3, 7, 10 (Sham-immunized n = 14, CTH522 immunized n = 12), 14 and 17 (Sham-immunized n = 8, CTH522 immunized n = 6). e Data points represent Log10 IFU/mouse and are presented as Box and Whiskers plots with median log10 IFU and 25th and 75th percentiles (Box) and Min to Max, all data points (Whiskers). f Kinetics of clearance, median log10 IFU/mouse at PID3-17 for CTH522/CAF®01 and Sham-immunized mice. g At PID10 (week 59) GT tissue were stimulated with CTH522 (n = 6). Non-infected mice were included as controls (n = 2). The percentages of cytokine positive (expression of any of the cytokines IFN-γ, IL-2, IL-17A, TNF-α) CD44highCD4+ iv- GT T cells out of all CD4+ iv- T cells were analyzed by flow cytometry. h Data points represent the percentage of IL-17A− (Th1) and IL-17A+ (Th17) out of CD4 iv- GT T cells producing the cytokine TNF-α, IL-2, and IFN-γ in any combination. b–d, f–h Each data point, bar, or line represents median levels with 25th and 75th percentiles. c, e, g Two-tailed Mann–Whitney U test. f Mixed-effect model for repeated measures. a Created with Biorender.com. b–h Source data are provided as a Source Data file.

Back to article page