Fig. 2: Epithelial Efhd2 deficiency exacerbates DSS-induced colitis.
Relative percentage change of body weight (a), DAI score (b), and representative images of colon morphology and lengths (c) of Efhd2f/f and Efhd2f/f Vil1cre/+ mice after 2.5% DSS administration for 6 days and sacrificed on day 7. Representative images of H&E (d) and periodic acid-Schiff (PAS) (e) staining of distal colon of Efhd2f/f and Efhd2f/f Vil1cre/+ mice subjected to DSS-induced colitis on day 0 and day 7. Left scale bar, 100 μm; right scale bar, 200 μm; arrows show intestinal goblet cells. f Histologic scores and PAS-positive cells per crypt in d, e. g Relative gene expression levels of Il6, Il1b and Il1a from colonic tissues of Efhd2f/f and Efhd2f/f Vil1cre/+ mice on day 7 after 6-day DSS treatment compared to day 0. h Representative images of E-cadherin (green) staining of the distal colon from Efhd2f/f and Efhd2f/f Vil1cre/+ mice after DSS administration on day 7. Scale bar, 150 μm. i Concentration of FITC-dextran from Efhd2f/f and Efhd2f/f Vil1cre/+ mice subjected to DSS-induced colitis was measured to detect intestinal permeability on day 0 and day 7. n = 6 per group (a–c, f, g, i). Data are representative of three independent experiments; error bars show means ± s.d. P values were determined by unpaired two-tailed t-test. For body weight curves, two-way ANOVA analysis with Sidak’s multiple comparisons test. Source data are provided as a Source Data file.
