Fig. 2: Peroxygenase-catalyzed hydroxylation of tetrahydrofuran. | Nature Communications

Fig. 2: Peroxygenase-catalyzed hydroxylation of tetrahydrofuran.

From: Reaction engineering blocks ether cleavage for synthesizing chiral cyclic hemiacetals catalyzed by unspecific peroxygenase

Fig. 2

a Reaction scheme. b Screening of immobilized enzymes under neat conditions. Conditions: [THF] = 0.5 mL (12.3 M), [immobilized rAaeUPO] = 100 mg (corresponding to 1.45 μM), [H2O2] = 6 mM h−1, 30 °C, 7 h. c Time course of the hydroxylation of tetrahydrofuran yielding tetrahydrofuran-2-ol (squares, 1a) and γ-butyrolactone (diamonds, 1b). Conditions: [THF] = 0.5 mL (12.3 M), [immobilized rAaeUPO] = 125 mg (corresponding to 1.81 μM), [H2O2] = 6 mM h−1, 30 °C, 24 h. The reported value is based on the mean value of two distinct experiments (n = 2).

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