Fig. 3: Spectral PRM cross correlation in the fingerprint and CH stretching regions.
a, b Raman signals from fat body tissues of young (n = 33) and old (n = 50) biologically independent Drosophila in the fingerprint and CH stretching regions. Error bands represent 1 standard deviation (SD) from the mean. c, d Raman signals of the cholesterol reference standard in fingerprint and CH stretching regions, respectively. e Fingerprint region similarity scores of young (n = 33) and old (n = 50) Drosophila fat body samples to the cholesterol reference standard. Box plots indicate median and interquartile range, with whiskers indicating ±1.5 times the interquartile range. p = 4.85 × 10−4 by two sided Wilcoxon rank sum test. f CH-stretching region similarity scores of young and old Drosophila fat body samples to the cholesterol reference standard. Box plots indicate median and interquartile range, with whiskers indicating ±1.5 times the interquartile range. p = 0.0037 by two sided Wilcoxon rank sum test. **p < 0.01; ***p < 0.001. PRM penalized reference matching. Source data are provided as a Source Data file.
