Fig. 4: PRM-SRS and fluorescence staining show similar results. | Nature Communications

Fig. 4: PRM-SRS and fluorescence staining show similar results.

From: Multi-molecular hyperspectral PRM-SRS microscopy

Fig. 4

a, b Comparison of PRM-SRS and fluorescence staining in control cells expressing shCtr (Ctr; a) and PGS1 knockdown (shPGS1; b) HEK293 cells. Panels on the top, two photon fluorescence microscopy (TPF) images following nonyl acridine orange (NAO)-labeling of CL. Panels on the bottom, label-free SRS hyperspectral images of CL at the CH-stretching region. c, d Quantitative analyses of NAO staining signal intensity and PRM-SRS imaging signal intensity (presented as similarity score in an 8-bit image) of CL in control (n = 31 cells over 2 technical replicates) and PGS1 knockdown (n = 32 cells over 2 technical replicates) cells. Significantly decreased signals in shPGS1 cells were detected by both TPF and PRM-SRS microscopy. Values are mean ± SEM. ****p < 0.0001 by Student’s t-test. n = 3 biologically independent experiments. Scale bar, 10 µm. TPF two photon fluorescence, NAO Nonyl-acridine Orange, CL Cardiolipin, shPGS1 short hairpin phosphatidylglycerophosphate synthase 1. Source data are provided as a Source Data file.

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