Fig. 1: AHL22 interacts with FRS7 and FRS12.
From: Chromatin attachment to the nuclear matrix represses hypocotyl elongation in Arabidopsis thaliana
a Bimolecular fluorescence complementation (BiFC) analysis of AHL22 and FRS7 or FRS12 interaction in planta. AHL22 and FRS7 or FRS12 fused with pSITE-nEYFP-N1 and pSITE-cEYFP-C1 vectors, respectively, were cotransformed into N. benthamiana. n = 3 independent experiments. Scale bar = 50 μm. b FRET-FLIM analysis of AHL22 and FRS7 or FRS12 interaction in planta. From top to bottom, representative images of nuclei, expresseing various GFP and/or RFP fused proteins in epidermal N. benthamiana cells, captured between wavelengths 540-545 nm and between 570-670 nm as well as the corresponding donor fluorescence lifetime calibrated to the adjacent color bar. The respective construct combinations are indicated. Scale bar = 10 um. c GFP donor fluorescence lifetime quantifications of the indicated construct combinations presented in a min to max box and whiskers plot showing the median (line in the middle of the box), mean (+ in the box), min and max (whiskers), individual points (gray circle) (n > =18). Statistical significance was calculated using a Welch’s Anova test combined with Dunnett’s T3 multiple comparisons test. P-values are represented as ****(p < 0.0001), ***(p < 0.001), **(p < 0.01). The experiment was performed in two independent infiltrations and the exact number of measurements is indicated per construct combination. d, e Co-immunoprecipitation assays showing interactions between AHL22 and FRS12 or FRS7. AHL22-3FLAG and FRS12-GFP or FRS7-GFP were expressed in N. benthamiana. WT, empty leaves. IP, immunoprecipitation. Asterisks indicate targeted proteins. n = 1 independent experiment.
