Fig. 3: AHL22 and FRS7/FRS12 co-repress hypocotyl growth.

a The hypocotyl phenotype of indicated lines grown vertically under LD conditions (16 h at 23 °C under 22 μmol·m⁻²·s⁻¹ continuous white light, 8 h at 23 °C dark) for 5 days. Scale bar= 1 cm. b Hypocotyl lengths of indicated lines grown under LD conditions (16 h at 23 °C under 22 μmol·m⁻²·s⁻¹ continuous white light, 8 h at 23 °C dark) for 5 days. The average length of three independent measurements ± standard deviations are shown. Each measurement with n = 30 plants. c Venn diagrams showing overlap of upregulated genes (UGs) in ahl22, frs7 frs12 and ahl22 frs7 frs12 compared with Col-0. d Heat map showing the relative mRNA expression levels of genes responsive to auxin in ahl22, frs7 frs12 and ahl22 frs7 frs12 Log2FC row scale was used. e The hypocotyl phenotype of indicated lines grown vertically under LD conditions (16 h at 23 °C under 22 μmol·m⁻²·s⁻¹ continuous white light, 8 h at 23 °C dark) for 5 days. Scale bar= 1 cm. f Hypocotyl lengths of indicated lines grown under LD conditions (16 h at 23 °C under 22 μmol·m⁻²·s⁻¹ continuous white light, 8 h at 23 °C dark) for 5 days. Naphthylphthalamic acid (NPA) is a key inhibitor of auxin transportation in plants. DMSO is the solvent for the NPA and included as a control. Average length of three independent measurements ± standard deviations are shown. Each measurement with n = 30 plants. Unpaired two-tailed Student’s t-test was used to determine significance. N.S. p value > 0.05, *p value ≤  0.05, **p value  ≤  0.01, ***p value ≤ = 0.001, ****p value ≤ = 0.0001.