Fig. 7: AHL22 recruits HDA15 to remove histone acetylation.
From: Chromatin attachment to the nuclear matrix represses hypocotyl elongation in Arabidopsis thaliana
a. Venn diagrams showing overlap of UGs and DGs in ahl22frs7frs12 and hda15-1. Significance was tested using a hypergeometric test. b Relative expression level of the selected genes by qRT-PCR from 5-day-old whole plants in hda15-1. Values are means ± SD of three biological repeats. The significance of differences at each gene was tested using one-way ANOVA with Tukey’s test (P < 0.05), and different letters indicate statistically significant differences. c Relative H3ac enrichment at selected genes determined by ChIP-qPCR in Col-0, triple and hda15-1 plants. Values are means ± SD of three biological repeats. The significance of differences at each gene was tested using one-way ANOVA with Tukey’s test (P < 0.05), and different letters indicate statistically significant differences. d IgG and HDA15-GFP enrichment levels at selected genes in indicated lines. The amounts of immunoprecipitated DNA fragments were quantified by qPCR and normalized to input DNA. Values are means ± SD of three biological repeats. The significance of differences at each gene was tested using one-way ANOVA with Tukey’s test (P < 0.05), and different letters indicate statistically significant differences. e Proposed working model. AHL22 interacts with FRS7 and FRS12 at the nuclear matrix and recruits chromatin regions and histone deacetylases, HDA15, to the nuclear matrix and silence the target genes by reducing H3ac level to repress hypocotyl growth.
