Fig. 6: GPR30 expressed in brain mural cells is activated by bicarbonate.
From: Bicarbonate signalling via G protein-coupled receptor regulates ischaemia-reperfusion injury
a–c Calcium mobilisation assay for endogenous GPR30 in primary culture. The primary culture of Venus-positive cells harvested from the brain cortex of Gpr30-heterozygous (a, Gpr30+/Venus) and Gpr30-null (b, Gpr30Venus/Venus) mice were subjected to calcium imaging. c Quantification of a) and b) as area under the curve. d–i Calcium mobilisation assay for freshly isolated SMCs (d–f) and pericytes (g–i). Freshly isolated mCherry-positive cells harvested from the cerebral arteries (d, e) and cortex (g, h) of Gpr30-heterozygous (d, g, Gpr30+/iCre; Rosa26-GCaMP6) and Gpr30-null (e, h, Gpr30iCre/iCre; Rosa26-GCaMP6) mice were subjected to calcium imaging. f, i Quantification of d and e, g and h, as area under the curve, respectively. Statistical analysis: two-tailed unpaired t-test with Bonferroni’s correction after two-way ANOVA (c, f, i). Data are presented as mean values ± SEM. P values are shown if significant. ns indicates no significant difference. Source data are provided as a Source Data file.
