Fig. 3: CD4 help-dependent intrahepatic expansion of CD8+ T cells is driven by local proliferation in portal tracts and PCV regions.
From: A hepatic network of dendritic cells mediates CD4 T cell help outside lymphoid organs
a–c Intrahepatic localisation of transferred gBT-1 T cells undergoing cell division (Ki67+) 12-days following rAAVgB-Ag85b treatment. Representative immunofluorescent (IF) images of livers from isotype control treated (top panels) and CD4 depleted (bottom panels) recipient mice showing the size and location of Ki67+ gBT-1 cell clusters (a) and quantification of the number of Ki67+ gBT-1 T cells in clusters localized to portal tracts (b) and peri-central venous (PCV) regions (c). d–h Intrahepatic gBT-1 T cell clusters detected in portal tracts and PCV regions are the result of local clonal expansion. Protocol (d) and representative images showing CD45.1+ and tdTomato+ gBT-1 T cell clones in the sinusoidal compartment (e), portal tracts (f) and PCV regions (g). Quantification (h) of the fraction of nearest non-self (tdTomato+) neighbours of CD45.1+ gBT-1 T cells in the sinusoidal compartment, portal tracts and PCV regions. Data are representative of two independent experiments. n = 5 mice per group (a–c) and n = 4 mice per group (d-h). Error bars indicate ± SEM. Analysed with a two-tailed unpaired Student’s t test (b, c) and Kruskal–Wallis test with multiple comparisons (h). Scale bars, 300 μm/50 μm (a), 40 μm (e), 20 μm (f, g). Source data are provided as a Source Data file.
