Fig. 4: Portal tracts and PCV regions support the transfer of CD4 help to intrahepatic CD8+ T cells.
From: A hepatic network of dendritic cells mediates CD4 T cell help outside lymphoid organs
a–c Total number of intrahepatic effector and memory gBT-1 T cells post-rAAV treatment of recipient mice administered isotype control, or in which CD4 T cells were depleted at day 0, 3 or 6 (a), or at day 0 or 9 (b, c). d–f Kinetics of adoptively transferred gBT-1 and P25 T cell numbers detected in parenchyma/sinusoidal (d), portal tract (e) and PCV (f) liver compartments following rAAV treatment of recipient C57BL/6 mice (red P25 T cells; black/grey gBT-1 T cells). g–i Representative IF images of gBT-1 and P25 T cells in the liver of rAAV treated animals 8- (g), 10- (h) and 12- (i) days post-rAAV treatment, showing that CD4+ T cells enter portal tracts and interact with cognate CD8+ T cells after day 8. Data are representative of two independent experiments. n = 4 (a), n = 5 (b, c), n = 4 (d–i) mice per group. Error bars indicate ± SEM. Analysed with Kruskal–Wallis test with multiple comparisons. Scale bars, (g) 100 μm/10 μm, (h) 100 μm/20 μm, (i) 150 μm/20 μm/20 μm. Source data are provided as a Source Data file.
