Fig. 3: Activated Wnt/β-catenin signaling in ILC3 increases susceptibility to colitis. | Nature Communications

Fig. 3: Activated Wnt/β-catenin signaling in ILC3 increases susceptibility to colitis.

From: Dysregulation of Wnt/β-catenin signaling contributes to intestinal inflammation through regulation of group 3 innate lymphoid cells

Fig. 3

a–j 8-week-old RorccreCtnnb1ex3fl/wt mice (n = 5) and their littermates Ctnnb1ex3fl/wt mice (n = 5) were given 3% DSS in drinking water for 5 days. a, b Body weight change (a) and survival rates (b) at different time points. c, d Gross morphological changes (c) and lengths of colons (d). e Histological analysis of representative colons. Scale bars represent 100 μm. f Colitis score of Ctnnb1ex3fl/wt and RorccreCtnnb1ex3fl/wt mice. g Flow cytometry analysis of colonic ILCs (gated in lineage–CD127+). h The percentage of colonic ILC3s in total CD45+ cells. i Flow cytometry analysis of cytokine production in colonic ILCs (gated in lineage–CD127+). Cytokine production was detected under PMA/Ionomycin treatment. j The percentage of IL-17A+ and IL-22+ cells in total colonic ILCs. k–n 8-week-old RorccreCtnnb1ex3fl/wt mice and their littermates Ctnnb1ex3fl/wt mice were given 2.5% DSS in drinking water for 5 days, followed by normal drinking water. Mice were divided into three groups: Ctnnb1ex3fl/wt mice (n = 8), RorccreCtnnb1ex3fl/wt mice with or without ILC3s replenishment (n = 6 per group). k–l Body weight change (k) and survival rates (l) at different time points post DSS treatment. m Gross morphological changes of colons from three groups. n Lengths of colons and colitis score from three groups (n = 6 per group). o–r 8-week-old Rag1–/–RorccreCtnnb1ex3fl/wt mice (n = 5) and their littermates Rag1–/–Ctnnb1ex3fl/wt mice (n = 5) were given 3% DSS in drinking water for 5 days. o, p Body weight change (o) and survival rates (p) at different time points post DSS treatment. q Gross morphological changes of colons. r Lengths of colons and colitis score. Each dot represents one individual mouse. Error bars represent the SEM. Statistical significance was tested by two-sided two-way ANOVA with Sidak correction adjusted for multiple comparisons (a, k, o), two-sided log-rank Mantel–Cox test (b, l, p), two-sided one-way ANOVA with Tukey’s test adjusted for multiple comparisons (n) and unpaired two-sided Mann–Whitney U test (d, f, h, j, r). Data are representative of two (k–r) or three (a–l) independent experiments.

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