Fig. 2: Nf1 is dispensable in myofibers.

a Quantitative real-time PCR for Nf1 (left) and Myog (right) on primary mouse myoblasts cultured in proliferation medium or upon myogenic induction for indicated time (n = 3 animals per genotype; each dot represents the mean of three technical replicates from one biological replicate; p-values shown). b Quantitative real-time PCR for Nf1 in p7 or p21 MPs, p21 whole muscle tissue or p21 muscle-derived fast-adhering fibroblastic cells (FBs) (n = 3 animals per genotype; each dot represents the mean of three technical replicates from one biological replicate; p-values shown). c Whole-body appearance of control and Nf1^Acta1 mice at p21. d Cross sections of lower hind limbs of control and Nf1^Acta1 mice at p21 immunolabeled for Laminin (green), TA Tibialis anterior, EDL Extensor digitorum longus. Magnifications of indicated areas in TA muscles shown right. e Quantification of cross-sectional area (CSA; left) and myofiber Feret’s minimum diameter (right) of control and Nf1^Acta1 TA and EDL muscles (n = 3 animals per genotype; p-values shown). f immunolabeling for Laminin (gray), MyHC-1 (red), MyHC-2A (purple), and MyHC-2B (green) on cross sections of TA (left) and EDL (right) muscles of control and Nf1^Acta1 mice at p21. g Quantification of fiber types in p21 control and Nf1^Acta1 TA and EDL muscles (n = 3 animals per genotype; p-values shown). Data are mean ± SEM; P-value calculated by two-sided unpaired t-test. Source data are provided as a Source Data file.