Fig. 5: Metabolic reprogramming of Nf1^Myf5 MPs.

a GSEA of control and Nf1^Myf5 p7 MP RNA-Seq data for “glycolysis - gluconeogenesis” and “oxidative phosphorylation.” b–d Heatmaps show significant DEGs in Nf1^Myf5 versus control MPs related to glycolysis and pyruvate dehydrogenase complex (b), TCA cycle components (c) and electron transport chain components (d). e SeahorseXF flux analysis of control and Nf1^Myf5 p7 MPs; quantification of ECAR (n = 3 independent biological replicates from 3 animals per genotype; each dot represents the mean of five technical replicates from one biological replicate; p-values shown). f SeahorseXF flux analysis of control and Nf1^Myf5 p7 MPs; quantification of OCR (n = 3 independent biological replicates from 3 animals per genotype; each dot represents the mean of five technical replicates from one biological replicate; p-values shown). g Venn diagram showing 130 commonly downregulated genes between Nf1^Myf5 p7 MPs and Nf1^Myf5 p21 muscle. GO analysis of commonly downregulated genes shown below. h Averaged normalized coverage for H4K16ac derived from ChIP-Seq on control and Nf1^Myf5 p7 FACS-isolated MPs. TSS, transcription start site. i Immunolabeling for Pax7 (green) and H4K16ac (red) on FACS-isolated cytospun MPs from p7 control and Nf1^Myf5 animals. Quantification of anti-H4K16ac relative fluorescence intensity (RFI) is shown right. Data range is shown as violin plot with median and interquartile range, means of biological replicates are shown as dots (n = 3 animals per genotype; p-value shown). j ChIP-Seq tracks for H4K16ac from control and Nf1^Myf5 p7 MPs at the Myh3 locus. Data are mean ± SEM; P-value calculated by two-sided unpaired t-test. Source data are provided as a Source Data file.