Fig. 6: Increased Notch signaling drives Nf1^Myf5 MPs to quiescence.

a RT-qPCR of Notch pathway component and target genes in RNA extracted from freshly FACS-isolated control and Nf1^Myf5 p7 MPs (n = 3 animals per genotype; each dot represents the mean of three technical replicates from one biological replicate; p-values shown). b RT-qPCR for Notch targets on FACS-isolated control or Nf1^Myf5 p14 MPs cultured on Matrigel without coating or Jagged-1 coating for 48 h (n = 3 independent experiments from 3 animals per genotype; p-values shown). c FACS-isolated control or Nf1^Myf5 p14 MPs cultured on Matrigel without coating or Jagged-1 coating for 48 h stained for Pax7 (green) and Ki67 (red). d Ki67⁺ cell quantification among Pax7⁺ cells on image data as in (c) (n = 3 animals per genotype; p-values shown). e Anti-Pax7 relative fluorescence intensity (RFI) quantification on image data as in (c). Data range is shown as violin plot with median and interquartile range, means of biological replicates are shown as dots (n = 3 animals per genotype; p-values shown). f Immunolabeling for MyoD (red) on FACS-isolated control or Nf1^Myf5 p14 MPs cultured on Matrigel w/o coating or Jagged-1 coating for 48 h. g Quantification of MyoD⁺ cells / total cells on image data as in (f) (n = 3 animals per genotype; p-values shown). h Quantification of anti-MyoD relative fluorescence intensity (RFI) on image data as in (f). Data range is shown as violin plot with median and interquartile range, means of biological replicates are shown as dots (n = 3 animals per genotype; p-values shown). i GSEA on RNA-Seq data from control and Nf1^Myf5 p7 MPs for “nitric oxide stimulates guanylate cyclase”. j RT-qPCR for Notch targets Pax7, Hes1 and Hey1 on FACS-isolated control or Nf1^Myf5 p14 MPs. MPs were cultured on Matrigel without coating or with Jagged-1 coating for 48 h, with or without Mek inhibitor UO126 or pan-NOS inhibitor L-NAME. Bars show fold-changes of Nf1^Myf5 MPs relative to control MPs, control MPs were set as 1 (n = 3 independent experiments from 3 animals per genotype; p-values shown). Data are mean ± SEM; P-value calculated by two-sided unpaired t-test. Source data are provided as a Source Data file.