Fig. 7: Rescue of Pax7 cell depletion, cell cycle exit, and metabolic reprogramming by Notch pathway inhibition.

a RT-qPCR of selected glycolysis, TCA, and OXPHOS genes on FACS-isolated WT p14 MPs cultured on Matrigel without coating or Jagged-1 coating for 48 h. Pax7, Hes1, and Hey1 tested as internal controls (n = 4 animals per condition; each dot represents the mean of three technical replicates from one biological sample; p-values shown). b Schematic depiction of DAPT treatment of Nf1^Myf5 animals. c Representative images of TA muscle sections of postnatal Nf1^Myf5 mice treated with placebo or DAPT, stained for Pax7 (red), Ki67 (green), collagen IV (gray), and DAPI (blue; nuclei). d p21 Pax7⁺ cell quantification in Nf1^Myf5 mice treated with placebo or DAPT (n = 4 animals per condition; p-values shown). e p21 Ki67⁺/Pax7⁺ cell quantification relative to Pax7⁺ cells in Nf1^Myf5 mice treated with placebo or DAPT (n = 4 animals per condition; p-value shown). f RT-qPCR for glycolysis, TCA, and OXPHOS genes on muscle tissue from Nf1^Myf5 mice treated with placebo or DAPT (n = 3 animals per condition; each dot represents the mean of three technical replicates from one biological sample; p-values shown). g Representative images of Laminin (green) immunolabeling on sections of Nf1^Myf5 mice treated with placebo or DAPT shown left. Right: distribution of myofiber diameter in Nf1^Myf5 mice treated with placebo or DAPT (n = 4 animals per condition). h Body weight of Nf1^Myf5 mice treated with placebo or DAPT (n = 4 animals per condition; p-value shown). i Posterior subcutaneous white adipose tissue weight in Nf1^Myf5 mice treated with placebo or DAPT (n = 4 animals per condition; p-value shown). Data are mean ± SEM; P-value calculated by two-sided unpaired t-test. Source data are provided as a Source Data file.