Fig. 2: Conservation of LDTGo.
A Phylogenetic tree showing the conservation and distribution of LD-transpeptidases. In green, LDTGo-like proteins (LD1,3-TPases) and in blue, homologs to YcbBEc (LD3,3-TPases). Homology was assessed by BLAST against the NCBI and OrthoDBv11 databases. B Comparison of the domains in representative LDTGo orthologues from diverse bacterial Families: WP_011252635.1 (Gluconobacter oxydans), WP_041249327.1 (Gluconacetobacter diazotrophicus), WP_124305792.1 (Acetobacter pasteurianus), WP_099541073.1 (Acetobacter pomorum), WP_253736052.1 (Granulibacter bethesdensis), WP_254845249.1 (Desulfovibrio sp. DV), EHJ47292.1 (Solidesulfovibrio carbinoliphilus), B4EIM9_BURCJ (Burkholderia cenocepacia), QFS41266.1 (Burkholderia cepacian), WP_244096448.1 (Burkholderia dolosa), AYZ63023.1 (Burkholderia multivorans), WP_215249195.1 (Variovorax paradoxus), ALX84167.1 (Achromobacter denitrificans), AOB33672.1 (Bordetella sp. H567). The presence of a signal peptide is indicated. Signal peptides and transmembrane domains are predicted using SignalP 6.0. C UV muropeptide profiles of the heterologous expression of LDTGo and its homologs from Acetobacter pasteurianus (LDTAp), Burkholderia cenocepacia (LDTBcn) and a catalytically inactive mutant (LDTBcn C354A) in E. coli BL21. LD1,3-crosslinked muropeptides are highlighted in green. D UV muropeptide profiles of G. oxydans (Go) ΔldtGo mutant and complemented derivatives expressing the LDTGo and LDTBcn. LD1,3-crosslinked muropeptides are highlighted in green.
