Fig. 7: DD-crosslinking turnover controls LD1,3-crosslinking levels. | Nature Communications

Fig. 7: DD-crosslinking turnover controls LD1,3-crosslinking levels.

From: A distinctive family of L,D-transpeptidases catalyzing L-Ala-mDAP crosslinks in Alpha- and Betaproteobacteria

Fig. 7

A Scheme of the production of monomeric substrates of LDTGo by DD-CPases (blue) and DD-endopeptidases (red). Cleavage sites are indicated by arrowheads. DD-crosslinking (B) and LD-crosslinking (C) levels for G. oxydans (Go) wild-type (WT), ΔldtGo, Δpbp7Go and the indicated complemented strains. Relative molar abundances of the DD- and LD1,3-crosslinked muropeptides were quantified and represented relative to the Go WT levels. D Representative images of G. oxydans WT and ΔldtGo treated with Ampicillin MIC test strips. E Table indicating MIC values for Ampicillin (Amp) and Ampicillin/Sulbactam (Amp+Sul) for the G. oxydans WT, ΔldtGo and ΔldtGo pLDTGo complemented strain. Error bars in graphs B, C represent standard deviation from mean. Significant differences (unpaired t-test, two-tailed) are indicated: *p-value < 0.05; **p-value < 0.001; ***p-value < 0.0001; ns: not significant. Source data for B, C and exact p-values are provided as a Source Data file.

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