Fig. 1: Characterization of LSC-Exo and surface expression of ACE2 on LSC-Exo.
From: Inhalation of ACE2-expressing lung exosomes provides prophylactic protection against SARS-CoV-2
a Extraction scheme of LSC and LSC-Exo from healthy donors, created with Biorender.com. b Immunofluorescence staining and quantification analysis of ACE2 on LSC and HEK. Scale bar: 50 μm. n = 3. c Western blot quantification of ACE2 expression in LSC and HEK, which derived from the same experiments and processed in parallel. n = 3. d Representative TEM images of LSC-Exo and HEK-Exo from 3 independent experiments. Scale bar: 100 μm. e Measurements of size distribution of LSC-Exo and HEK-Exo via nanoparticle tracking analysis. Inset: 3-colar dSTORM image of CD63-Alexa Fluor®-488, PE-CD9, APC-CD81 of LSC-Exo or HEK-Exo. f Quantification of ACE2 expression on LSC-Exo and HEK-Exo by flow cytometry. n = 3. Gating strategy was shown in Fig. S20a. g Western blot and quantification analysis of ACE2 levels on LSC-Exo and HEK-Exo. This quantification analysis derived from the same experiments. n = 3. Data are mean ± s.d. A two-tailed, unpaired Student’s t-test was performed for statistical analysis. Source data are provided as a Source Data file.
