Fig. 4: EP-Seq and sequence conservation analysis reveal functional sites of DAOx.
From: Understanding activity-stability tradeoffs in biocatalysts by enzyme proximity sequencing
A The distance to the catalytic site for positions that are evolutionarily conserved (conservation score, CONS = 100) were plotted as a function of EP-Seq expression scores (average per position) and analyzed through Pearson linear correlation function with a two-sided statistical test. The nine highly conserved residues associated with the most positive expression scores (light gray right corner) are visualized in (B). B Structural details of DAOx residues with CONS = 100 and expression score > 0. H-bonds are shown in green. The FAD cofactor and D-alanine carbon backbones are colored in yellow and green, respectively. DAOx residue carbon backbones are colored in light blue with colored elements: Hydrogen (white), Nitrogen (dark blue), Oxygen (red), Phosphorus (orange). C Expression and activity scores for each of the conserved positions linked to positive expression scores.
