Fig. 5: EP-Seq predicts activity enhancing mutations in DAOx.
From: Understanding activity-stability tradeoffs in biocatalysts by enzyme proximity sequencing
A Average expression and activity fitness scores per position in the DAOx enzyme. The coloring indicates relative distance to the active site. B Normalized activity values are depicted on the 3D structure of a DAOx monomer (right). Areas of the enzyme where mutations on average led to increased activity are represented in green, while regions where mutations on average had a negative impact on activity are shown in red. The most frequent 6 positions among the top 1000 mutants with highest normalized activity scores are indicated.
