Fig. 1: SEL1L^S658P KI mice exhibit partial embryonic lethality and developmental delay.

a Gross appearance of SEL1L^S658P KI embryos (one dead, right) compared with their WT littermates at E10.5. b Growth curve. n = 12, 28 and 12 mice for male WT, HET and KI; n = 12, 22 and 11 mice for female WT, HET and KI. c Body length of 5-week-old mice of both sexes (n = 6, 4 and 7 mice for WT, HET and KI). d 72 h food intake of 5-week-old mice of both sexes (n = 3 mice per group). e, f Hematoxylin & eosin (H&E) images of various peripheral tissues (e, pancreatic acini and islet; f, Liver, iWAT and BAT) from 5-week-old mice (n = 3 mice per group). iWAT, inguinal white adipose tissue; BAT, brown adipose tissue. g Serum levels of alanine transaminase (ALT), total bilirubin (TBIL) and alkaline phosphatase (ALP) in 5-week-old mice of both sexes (ALT: n = 7, 8, 9 and 12 mice for female WT, KI, male WT and KI; ALP: n = 8, 8, 9 and 12 mice for female WT, KI, male WT and KI; TBIL: n = 8, 7, 7 and 12 mice for female WT, KI, male WT and KI). h Serum levels of blood urea nitrogen (BUN) and creatinine (CREA) in 5-week-old mice of both sexes (BUN: n = 9, 12, 8 and 8 mice for female WT, KI, male WT and KI; CREA: n = 8, 8, 9 and 12 for female WT, KI, male WT and KI). Values, mean ± SEM. n.s. not significant; **p < 0.01, ***p < 0.001 and ****p < 0.0001 by two-way ANOVA followed by Tukey’s multiple comparisons test (b), one-way ANOVA followed by Tukey’s post hoc test (c, d) and two-tailed Student’s t test (g, h).